Association of Calpain-10 gene polymorphisms with Type 2 diabetes mellitus: a case-control study from a tertiary care hospital in Pakistan

Table 1 Primer sequences and restriction enzymes used for the analysis of CAPN-10 gene polymorphisms

SNP	Primer Sequence 5’ → 3’	PCR Product	Restriction Enzyme	Restriction Product (bp)
SNP-43 (rs3792267) G→A	F: gct ggc tgg tga cat cag tgc	245 bp	NdeI	GG: 245 GA: 245, 223, 31 AA: 223, 31
SNP-43 (rs3792267) G→A	R: acc aag tca agg ctt agc ctc acc ttc ata	245 bp	NdeI	GG: 245 GA: 245, 223, 31 AA: 223, 31
SNP-44 (rs2975760) T→C	F: gca ggg cgc tca cgc ttg ccg	166 bp	BstUl	TT: 166 TC: 166, 145, 21 CC:145, 21
SNP-44 (rs2975760) T→C	R: gca tgg ccc cct ctc tga ttc	166 bp	BstUl	TT: 166 TC: 166, 145, 21 CC:145, 21

SNP: Single Nucleotide Polymorphism; PCR: Polymerase Chain Reaction; bp: Base Pair; NdeI: A restriction enzyme used for digesting DNA at specific sites; BstUI: A restriction enzyme used for DNA fragment analysis

ISSN: 1755-8794