Fig. 1

Steps of PCR and genotyping for CAPN-10 polymorphisms. Schematic representation of the four-step process used to genotype SNP43 and SNP44: (1) DNA extraction from whole blood, (2) PCR amplification of target regions, (3) enzymatic digestion with NdeI (SNP43) and BstUI (SNP44), and (4) gel electrophoresis for genotype identification based on fragment size