Fig. 6
Knockdown of demethylases in 1ry cultured NK cells doesn’t regulate mRNA expression of target genes. A Western blot analysis of a demethylase knockout in NK cells from a representative trial. ERK was used as a loading control. (Different lanes from the same gel are shown. Full-length blots are provided in the Supplementary file). B Quantification of demethylase protein expression using ImageJ after four knockout trials, assessed seven days post-knockout to test knockout efficiency. Data is presented as mean ± SD. C RT-qPCR analysis of mRNA levels of target genes in demethylase-knockdown cells compared to control untransfected cells (electroporated without synthetic guide RNAs). Data is presented as mean ± SD (n = 2). To measure relative mRNA expression levels, RPLP0 was used as an internal control (the housekeeping gene) to normalize the data across different samples. Differences were analyzed using an unpaired Student’s t-test to compare the two groups