Fig. 4
Increasing N6-methyladenosine (m6A) by MA decreases mRNA expression of target genes in 1ry cultured NK cells. A MeRIP-RT-qPCR analysis of mRNA enrichment of the target genes in MA-treated cells compared to DMSO-treated cells. mRNA enrichment in DMSO-treated cells was set to 1. Data is presented as mean ± SD from 3 independent subjects (n = 3). CT values were normalized to an exogenously added m6A-modified control (Gaussia luciferase), provided in the EpiMark® N6-methyladenosine Enrichment Kit (NEB, MA, USA). This RNA m6A-modified control was spiked into the samples before RNA fragmentation to monitor m6A enrichment. B RT-qPCR analysis of mRNA expression of target genes in MA-treated cells vs DMSO-treated cells. Data is from six different donors and is presented as mean ± SD (n = 6). To measure relative mRNA expression levels, RPLP0 was used as an internal control (the housekeeping gene) to normalize the data across different samples. Differences were analyzed using an unpaired Student’s t-test to compare the two groups