Fig. 5

Activation of Wnt/β-catenin pathway partially counteracts the effect of LIMA1 on BCa cells. (A) MTT assay was used to detect the cell survival rate of J82/CR cells in vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group under cisplatin treatment, and IC50 was calculated; (B) Colony formation assay was used to detect the proliferation ability of J82/CR cells in vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group; (C) Transwell assay was used to detect the migration ability of J82/CR cells in vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group; (D) Transwell assay was used to detect the invasion ability of J82/CR cells in vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group; (E) Western blot assay was used to detect protein levels of E-cadherin, N-cadherin, and Snail in J82/CR cells of vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group, with representative bands provided; (F) Results of statistical analysis of protein levels of E-cadherin, N-cadherin, and Snail in J82/CR cells of vector group, LIMA1 group, LiCl group, and LIMA1 + LiCl group. N = 3; **P < 0.01 vs. vector group; ##P < 0.01 vs. LIMA1 group