Table 1 Primer sequences used in relative comparative real-time PCR
From: MEIS1 knockdown upregulates WNT signaling pathway genes in esophageal squamous cell carcinoma
Gene | Forward primer | Reverse primer | Amplicon size |
|---|---|---|---|
MEIS1 | ATGACACGGCATCTACTCGTTC | T GTCCAAGCCATCACCTTGCT | 105 |
FZD1 | CTCCTACCTCAACTACCACTTC | CACTGACCAAATGCCAATCC | 136 |
LRP5 | CGGCAGAAGGTGGTGGAG | CAGCGAGTGTGGAAGAAAGG | 212 |
DVL1 | TGGTCTCCTGGCTGGTCCTG | TCCGTGCCTGTCTCGTTGTC | 184 |
APC | AGACTGGTATTACGCTCAACTTC | CTGGCTATTCTTCGCTGTGC | 195 |
GSK3β | ACTTCACCACTCAAGAACTGTCAAG | TGTCCACGGTCTCCAGTATTAGC | 139 |
FBXW7 | CAGCAACAGCAACTCAGACAAC | CCTCCTCCTCATCCTCCTCATC | 226 |
LEF1 | GACAAGCACAAACCTCTCAG | TTATTTGATGTTCTCGGGATGG | 202 |
TCF7 | GGACAACTACGGGAAGAAGAAG | TAGAGCACTGTCATCGGAAGG | 138 |