Fig. 6
From: TPX2 promotes papillary renal cell carcinoma progression by forming a ceRNA with LINC00894
LINC00894 and miR-660-5p Regulate TPX2 Expression. A Predicted binding sites of TPX2 and LINC00894 with miR-660-5p. Dual luciferase reporter assays in HEK-293 cells cotransfected with reporter vectors and miR-660-5p mimics. B Western blot analysis of TPX2 protein levels in Caki-2 and ACHN cells transfected with miR-660-5p mimics or inhibitor. C Laser scanning confocal microscopy (LSCM) images showing miR-660-5p’s effect on mitotic spindle formation. D Representative images of subcutaneous xenograft tumors in nude mice injected with Caki-2 cells and treated with miR-660-5p agomir (2.5 nmol) every 3 days for 20 days. Statistical significance is denoted as *p < 0.05, **p < 0.01, and ***p < 0.001