Fig. 3
From: TPX2 promotes papillary renal cell carcinoma progression by forming a ceRNA with LINC00894
Oncogenic Role of TPX2 in Proliferation, Migration, and Invasion In Vitro and In Vivo. A Western blot analysis of TPX2 protein levels in Caki-2 and ACHN cells transfected with scrambled shRNA (shSCR), TPX2 shRNA (shTPX2), empty vector, or TPX2 overexpression plasmid (OE-TPX2). B Cell proliferation assay showing relative proliferation rates after 96 h. C Colony formation assay and quantification of colonies formed by shSCR, shTPX2, vector, and OE-TPX2 cells. D Wound healing assay demonstrating reduced migration in TPX2-knockdown cells (scale bars = 100 μm). E Transwell invasion assay showing decreased invasion in TPX2-knockdown cells (scale bars = 20 μm). F Representative images and quantification of tumor xenografts in nude mice (n = 5) from shSCR, shTPX2#1, vector, and OE-TPX2 groups. Statistical significance is denoted as *p < 0.05, **p < 0.01, and ***p < 0.001