Table 1 Primer sequences for RT-PCR
From: Bioinformatics identification and validation of pyroptosis-related gene for ischemic stroke
Gene | Sequences (5’-3’) |
|---|---|
GAPDH | F: CCTCGTCCCGTAGACAAAATG; R: TGAGGTCAATGAAGGGGTCGT |
Irf1 | F: ACCCTGGCTAGAGATGCAGATTA; R: TTGTTGATGTCCCAGCCGT |
Icam1 | F: GTACTGTACCACTCTCAAAATAACTGG; R: TGGGGCTTGTCCCTTGAGT |
Tlr2 | F: CCAAAGTCTAAAGTCGATCCGC; R: AGCCCATTGAGGGTACAGTCGT |
TNF | F: CCCTCACACTCACAAACCACC; R: CTTTGAGATCCATGCCGTTG |
Cebpb | F: TGGACAAGCTGAGCGACGAG; R: TTGAACAAGTTCCGCAGGGT |
Il1rn | F: CTCTCCTTCTCATCCTTCTGTTTCA; R: TCCTTGTAAGTACCCAGCAATGAG |
casp8 | F: CTTGAAGGAAGGGAAGAGTTGC; R: CACTGTCTTGTTCTCTTGGCGA |
Caspase-1 | F: GGCTGACAAGATCCTGAGGG; R: TAGGTCCCGTGCCTTGTCC |
IL-1β | F: GTAATGAAAGACGGCACACCC; R: CAGGCTTGTGCTCTGCTTGTG |
IL-18 | F: CCATGTCAGAAGACTCTTGCGT; R: CAAAGTTGTCTGATTCCAGGTCTC |
NLRP3 | F: ATGACTTTCCAGGAGTTCTTCGC; R: CCAAAGAGGAATCGGACAACAA |
GSDMD | F: AGTGCTCCAGAACCAGAACCG; R: TCACCACAAACAGGTCATCCC |
ASC | F: CTATCTGGAGTCGTATGGCTTGG; R: CAAAGTGTCCTGTTCTGGCTGT |