Fig. 5
From: USP2 reversed cisplatin resistance through p53-mediated ferroptosis in NSCLC
USP2 stabilized p53 protein by suppressing its ubiquitination. (A) Ubiquitination Assay was utilized to measure p53 ubiquitination after USP2 overexpression. (B) COIP was utilized to detect the binding relationship of USP2 and p53. (C) The p53 ubiquitination levels were assessed after mutation at the K305R, K372R or K382R sites in A549/DDP and H1299/DDP cells. (D) Western blotting was utilized for detecting the nucleus and cytoplasm p53 expression after mutation at the K305R, K372R or K382R sites in A549/DDP and H1299/DDP cells