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Table 2 Comparison of PGT and niPGT-A using TE, ICM and SCM biopsy samples from 10 donated blastocysts (positive in the 1st TE sample PGT)

From: Noninvasive preimplantation genetic testing for aneuploidy using blastocyst spent culture medium may serve as a backup of trophectoderm biopsy in conventional preimplantation genetic testing

Embryo

TE1st

TE1st

TE2nd

TE2nd

ICM

SCM

niPGT

EM6

46,XY, -11p (18 M, ×1)

FP

45,XY, -1q(70 M,×1,mos),+7p(×3,mos), -11(×1)

FP

46,XY

46,XY

TN

EM81

47,XX,+15

TP

46,XX,- X(×1, mos), -20q(×1, mos)

TP

46,XX, X(×1,mos), -15(×1, mos), -20q(×1)

46,XX

FN

EM91

46, XX, -11q(12 M, ×1)

FP

46,XX

TN

46,XX

46,XX

TN

EM98

46, XX, + 4(120 M, ×3)

FP

46,XX

TN

46,XX

46,XX

TN

EM26

46,XY, -5(1, mos),+18(×3,mos)

FP

46,XY,+2q(76 M,×3,mos)

FP

46,XY

46,XY

TN

EM37

46,XY, -8q(×1, mos)

FP

46,XY

TN

46,XY

46,XY

TN

EM53

46,XX,+22(×3,mos), +8q(×3, mos)

FP

46,XX

TN

46,XX

46,XX

TN

EM55

46,XX,+5p(×3,mos)

FP

46,XX

TN

46,XX

46,XX

TN

EM64

50,XY,+1(×3),+9(×3),+13(×3),+18(×3)

TP

46,XY, -13q(32 M, ×1,mos)

TP

46,XY,+8q(55 M,×3,mos)

46,XY

FN

EM35

46,XX,+6p(28 M,×3,mos)

TP

46,XX

FN

46,XX,+Xq(52 M,×3,mos),+21(×3,mos)

46,XX

FN

  1. TP: true positive; TN: true negative; FP: false positive; FN: false negative