Fig. 7

Nfe2l2 activation inhibited ferroptosis injury post tMCAO in mice. (A, B) TFR1, COX2 and FTH1 protein levels were determined through western blotting (n = 4). (C) Ferroptosis-related mitochondrial morphology was analyzed through transmission electron microscopy. White arrows indicate neuronal outer mitochondrial membrane rupture and mitochondrial cristae disappearance or reduction. Scale bar = 2 μm/1 µm. (D, E) DAPI (blue)/ROS (green) immunofluorescence images and statistical analyses on mouse brain tissue sections post-tMCAO. n = 3. Scale bar = 100 μm. The findings indicate means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 and N.S. nonsignificant versus sham group