Whole-exome sequencing uncovers the genetic basis of hereditary concomitant exotropia in ten Chinese pedigrees

BMC Medical Genomics

Table 2 Lists the Sanger sequencing-verified genes that achieved coisolation in each pedigree (including primers, product size, and annealing temperature)

Family ID	Gene	Primer sequence	Product size (bp)	Tm (°C)
F1	COL4A2	F: TGTGGGTTGGGAAGAGAACG	725	56.39
		R: GCAGCTCAGATGTTTCGCTG		59.87
F2	HERC2	F: TGGGGGAAAGAACCAACTCG	638	56.32
		R: ACACGTGGAAGCAGTAAGCA		57.51
F3	SYNE1	F: CACGGCTCAAAGTACACAAGT	374	55.61
		R: CATAGACTCTGAGCAGGCACT		57.57
F4	AUTS2	F: TATGCCACACTCGCATGTCA	543	55.4
		R: GAGCAGGCCACTCACCTTAAA		57.57
F5	CDH3	F: GAGTGGTTAAGGGACTCGCC	639	56.91
		R: GACTCATAGCCTGTCTCCGC		58.66
F6	SYNE1	F: TGAATGAAACCACCGCTCAGT	466	55.61
		R: CAGGAAATCCATGAAGGCTGG		57.57
F7	LOXHD1	F: ATCACCTTGGGAAGGGATCA	757	55.4
		R: GGACCCCATGAAGTTCTCAGT		57.57
F8	COL4A2	F: ACACTGACTTGCAGGGTAGC	616	57.18
		R: CTAGCCTGGCCCCAACTAAG		58.36
F9	COL4A2	F: CCTCTCTGGCATGGGTCAC	739	56.75
		R: CAGAGCACTAGGACCTGGGAA		58.93
F10	GTDC2	F: CCCCTAGGCGAGGAGTACAT	586	59.5
		R: CTGTCCACTTCTGCTTCCGT		57.45

ISSN: 1755-8794