Table 1 Primer and probe sequences for high priority pathogen qPCR assays
From: Development of a clinical metagenomics workflow for the diagnosis of wound infections
Assay target | Forward primer (5’-3’) | Reverse primer (5’-3’) | Probe sequence (5’-3’) | Source |
|---|---|---|---|---|
E. coli 16Â S rRNA gene | CATGCCGCGTGTATGAAGAA | CGGGTAACGTCAATGAGCAAA | TTCAGCGGGGAGGAAGGGAGTAAAGTTAATA | [41] |
P. aeruginosa oprL gene | CAGGTCGGAGCTGTCGTACTC | ACCCGAACGCAGGCTATG | AGAAGGTGGTGATCGCACGCAGA | [42] |
S. aureus protein coding sequence (NCBI Protein accession number: YP_500811.1) | AACTACTAGGGGAGCCTAATRAT | GGTACTAACCAAATCAGGTCATAA | TGGCTGAGATGAAYTGTTCAGACCC | [43] |
S. pyogenes speB gene | CTAAACCCTTCAGCTCTTGGTACTG | TTGATGCCTACAACAGCACTTTG | CGGCGCAGGCGGCTTCAAC | [44] |