Fig. 5

pcMINI vector construction for the gene ZNF462 c.6696–2 A > C. Minigene construction strategy. B Agarose gel electrophoresis revealed the presence of a single band corresponding to the wild type, and three distinct bands corresponding to the mutants b, c, and d. C Splice variant sequencing results in HeLa and 293T cells, with a wild type in the splice band, b: Intron8 right side lagging 116 bp, c: Exon 9 left side deletion of 26 bp, and d: Exon 9 jumping. Red * indicates the position of the variant