Table 1 Primer sequences used to analyze the variant of the COL4A2 gene and vector pcDNA3.1
Primer name | Primer sequence (5` − 3`) |
|---|---|
120,011-COL4A2-F | CGTTGCGGGTGTGTCTTCTC |
120,334-COL4A2-F | CCCAGGTGAAGCAGAACTTT |
122,754-COL4A2-R | GGATGACCGGACGATGGTCC |
123,097-COL4A2-R | ATATGCCTGCTAGCATGGGT |
pcDNA3.1-COL4A2-KpnI-F | GCTTGGTACCATGGGACACCCGGGGCAAGGTGG |
COL4A2-mut-F | ACAGATATCGGGTACATTTGCAAGAGATGGG |
COL4A2-mut-R | CCCATCTCTTGCAAATGTACCCGATATCTGT |
pcDNA3.1-COL4A2-EcoRI-R | TGCAGAATTCCTGGAAACCGACCAATCCAG |
pcDNA3.1-F | CTAGAGAACCCACTGCTTAC |
pcDNA3.1-R(BGH-R) | TAGAAGGCACAGTCGAGG |