Fig. 5
From: Functional analysis of a novel nonsense PPP1R12A variant in a Chinese family with infantile epilepsy
In-vitro functional analyses. (A) WB analysis of N-terminal FLAG-tagged wild-type PPP1R12A (FLAG-PPP1R12A) and variant (FLAG-PPP1R12A-Arg845X) proteins, revealing a decrease in the molecular weight of the variant protein, indicating the generation of a truncated protein. (B) Co-IP analysis revealing that Co-IP of FLAG-PPP1R12A-Arg845X with Myc PPP1CB produced no bands, indicating an effect of the mutation on the interaction between these two proteins