Fig. 3
From: Validating the splicing effect of rare variants in the SLC26A4 gene using minigene assay
The effect of the variant c.2089 + 3 A > T on splicing by minigene experiment. (A) Design of minigene experiment and transcript products in c.2089 + 3 A wild type and c.2089 + 3 A > T; (B) The gel electrophoresis of RT-PCR products using pMini-F/R primers showed a band of 444 bp in the c.2089 + 3 A wild-type and a band of 389 bp in the c.2089 + 3 A > T; (C) Sanger sequencing results of 444 bp and 389 bp PCR products showed two different splicing pattern