Fig. 2

Transcriptional dynamics during the transition from OP to DP. (A) Volcano plot displaying significant DEGs between DP and OP, with top genes labeled. (B) Clustering analysis of DEGs between DP and OP. (C) Top Gene Ontology (GO) terms enriched among up- and down-regulated genes between DP and OP. (D) Gene Set Enrichment Analysis (GSEA) delineates pivotal pathways modulated by DEGs between the DP and OP. (E) The interactions of the top 30 up-regulated and down-regulated DEGs were constructed using STRING (https://cn.string-db.org/) and visualized with Cytoscape. The colors represent the log2 fold change of DEGs (DP vs. OP). (F) Validation of DEGs during the transition from OP to DP by qPCR, with 12 samples in the OP group and 10 samples in the DP group (DP vs. OP, Student’s t-test). (G) Expression of CD83 and NR4A1 between the OP group (n = 12) and normal group (n = 12) was measured using qPCR (OP vs. Normal, Student’s t-test). OP: oliguric phase; DP: diuretic phase; DEG: differentially expressed gene. Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001