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Fig. 1 | BMC Medical Genomics

Fig. 1

From: Revealing differential expression patterns of piRNA in FACS blood cells of SARS-CoV−2 infected patients

Fig. 1

Fluorescence activated cell sorting of erythrocytes, monocytes, lymphocytes, neutrophils, basophils and eosinophils from peripheral blood. Purity verification of sorted cell populations. Panel (A) Erythrocytes were gated by CD235. Panel (B) Cell sorting purity control (Erythrocytes 99.12%). Panel (C) Lymphocytes and monocytes before sorting. Gating CD45+, low SSC. Panel (D) Gate CD45+, low SSC. Separation gates of lymphocytes and monocytes before sorting. Panel (E) Lymphocytes cell purity control (98.21%). Panel (F) Monocytes cell purity control (97.76%). Panel (G) All leukocytes gating CD45+ (Gate A). Panel (H) Gating eosinophils + basophils (CD45 + CD294 + lin-). Panel (I) Distinguishing eosinophils and basophils. Panel (J) Gating neutrophils by CD45 + CD15 + NOT 294+. Panel K. CD294 + cell purity control (95.24%). Panel L. Eosinophils cell purity control. Panel M (99,37%). Basophils cell purity control (99,87%). Panel N. Neutrophils (CD15 + CD294-) cell purity control against gate CD294+ (99,52%)

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BMC Medical Genomics

ISSN: 1755-8794

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