Fig. 4

The regulatory influence of CHMP4C on the cell cycle function in PCa cells. (a) GSEA functional analysis indicated the involvement of CHMP4C in the cell cycle of PCa. (b, c) Flow cytometry was utilized to assess alterations in the cell cycle of LNcaP and PC3 cells subsequent to CHMP4C knockdown. (d) The CCK-8 assay was used to determine the IC50 of LNcaP and PC3 cells treated with berberine for 24 h. (e, f) After treating LNcaP and PC3 cells with berberine at the IC50 concentration for a duration of 24 h, the mRNA and protein expression levels of CHMP4C were evaluated using qRT-PCR and Western blotting in LNcaP and PC3 cells