Fig. 2

Effects of CHMP4C on the proliferation, migration, and invasion abilities of PCa cells. (a, b) qRT-PCR and western blotting were used to analyze the mRNA and protein expression levels of CHMP4C after CHMP4C-sh1, CHMP4C-sh2, and CHMP4C-nc transfected LNcaP cells. (c, d) qRT-PCR and western blotting were used to analyze the mRNA and protein expression levels of CHMP4C after CHMP4C-sh1, CHMP4C-sh2, and CHMP4C-nc transfected PC3 cells. (e, f) The CCK-8 assay was used to evaluate the cellular proliferation of LNcaP and PC3 cells following CHMP4C knockdown. (g, h) The wound healing assay was employed to evaluate the migratory capability of LNcaP and PC3 cells subsequent to CHMP4C knockdown. (i, j) Transwell experiments were used to assess the migration and invasion capacities of LNcaP and PC3 cells following CHMP4C knockdown