Genetic evidence for predisposition to acute leukemias due to a missense mutation (p.Ser518Arg) in ZAP70 kinase: a case-control study

Table 3 PCR thermal-cycling parameters for sanger sequencing

Cycles	Step	Temperature	Time
1 cycle	Initial Denaturation	94 °C	5 min
	Denaturation	95 °C	15 S
35 cycle	Annealing:
	rs104893674	60 °C	30 S
	Extension	72 °C	45 S
	Final Extension	72 °C	10 min
	Hold	4 °C

ISSN: 1755-8794