Fig. 1

Summary of work flow for MPRA. Once candidate SNPs are identified, they are cloned into the center of 200 bp oligonucleotides (“oligos”) with the cognate flanking sequences and a weak upstream minimal promoter along with a downstream reporter (green fluorescence protein-GFP). After quality control measures to assure that each SNP is represented by a sufficient number of bar codes, oligo libraries are transfected into cells. After incubation with cells, GFP RNA is collected and sequenced. Bar codes allow for the quantification of allele-specific expression levels