Molecular genomic and epigenomic characteristics related to aspirin and clopidogrel resistance

BMC Medical Genomics

Table 4 Hierarchical linear regression analysis to predict PRU level

Variable groups and model performance	Variables	Model 1				Model 2
Variable groups and model performance	Variables	B	β	t value	VIF	B	β	t value	VIF
	Constant	172.136		7.413***		94.755		1.656†
Risk factors/blood tests	Sex (women)	25.070	0.221	5.797***	1.536	24.392	0.215	6.004***	1.600
	Age	0.650	0.111	3.105**	1.365	0.791	0.136	4.010***	1.431
	Creatinine	19.173	0.098	2.455*	1.685	15.468	0.079	2.088*	1.790
	Platelet	-0.060	-0.066	-1.916†	1.247	-0.078	-0.085	-2.629**	1.305
Single nucleotide polymorphisms	CYP2C192* (rs4244285) A/A					56.781	0.301	9.661***	1.210
	CYP2C192* (rs4244285) G/A					29.197	0.265	8.801***	1.132
	CYP2C193* (rs4986893) A/A					70.87	0.102	3.486***	1.066
	CYP2C193* (rs4986893) G/A					30.385	0.218	7.348***	1.098
	PGIR (rs4987262) G/A					20.862	0.085	2.907**	1.057
Promoter methylation	COX2					-1.597	-0.081	-2.206*	1.691
Promoter methylation	TXAS1					1.771	0.075	2.308*	1.333
Model summary	r	0.293				0.503
	R²	0.086				0.253
	Adjusted R²	0.068				0.210
ANOVA	F value	4.779***				5.959***

Model 1 = Regression analysis performed with variables including cardiovascular risk factors + blood tests
Model 2 = Regression analysis with variables including cardiovascular risk factors + blood tests + mediators + single nucleotide polymorphisms + promoter methylation
B Unstandardized coefficient, β Standardized coefficient, †< 0.1; *p < 0.05; **p < 0.01; ***p < 0.001
Reference group to create dummy variables: Sex = men; CYP2C19*2 (rs4244285) = G/G; CYP2C19*3 (rs4986893) = G/G; PGIR (rs4987262) = G/G.
ANOVA Analysis of variance, VIF Variance inflation factor
COX2 Cyclooxygenase 2 gene, CYP2C19 Cytochrome p450 2C19, PGIR Prostaglandin I receptor gene, TXAS1 Thromboxane A synthase 1 gene

ISSN: 1755-8794