Fig. 8

Immunohistochemistry and experimental verification. a The protein expression of COL4A1 and COL4A2 in immunohistochemical images between normal and tumor tissues. b-g The mRNA expression levels of COL4A1 (b), COL4A2 (c), COL4A3 (d), COL4A4 (e), COL4A5 (f) and COL4A6 (g) in GES-1, HGC-27 and MKN-45 were measured by qPCR. The results were normalized to the reference gene GAPDH. h Western blot protein detection of the COL4A1 and COL4A2 expression levels in cell lines. Images of blots intercepted from different parts of the same print, or from different blots separated by dividing lines. ^*P < 0.05; ^**P < 0.01; ^****P < 0.0001; ns P > 0.05