Fig. 3

Pathological examination. a HE staining of liver biopsy tissue (20 ×). b At high magnification, an isolated arteriole is observed (400 ×). c CK7 showed slight proliferation of fine bile ducts (40 ×). d PAS staining of liver biopsy tissue suggested a large deposition of glycogen in hepatocytes (100 ×). e–f Gross image of the surgically resected liver. g-i HE staining of the adenoma tissue showed that the liver cells were arranged regularly against the background of fatty liver without atypia, the boundary between the normal liver tissue and the adenoma was clear, and the mallory body could be seen at high magnification (20 × -400 ×). j IHC showed positive CD34 staining of adenoma vascular endothelial cells, indicating incomplete vascularization (40 ×). k IHC showed negative GS staining in the adenomas (40 ×). l Ki-67 staining showing a proliferative rate of less than 2% in adenoma tissue (40 ×). m IHC showed no absence of L-FABP in the adenomas (40 ×). n IHC showed that beta-catenin was not activated in the adenomas (100 ×). o IHC shows that CRP is strongly and diffusely positive in adenomas (lower right) but not in normal liver tissue (upper left) (100 ×). p Foot’s staining methods showed that reticulin was not lost (100 ×). q Masson staining showing wide fibrous septa between normal liver tissue and adenoma tissue (40 ×). r HE staining of surrounding liver tissue (100 ×). s PAS staining of the specimen showed a large amount of glycogen deposition in the surrounding liver tissue (100 ×). t PAS-stained hepatocytes that were mostly digested by PAS-D (100 ×)